By Bancroft W. D.
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Samples were placed between two A g C l windows, and s e a l e d w i t h a 6 uM spacer. The assembly was wrapped w i t h T e f l o n tape, as a f u r t h e r s e a l a g a i n s t d e h y d r a t i o n , and i n s e r t e d i n t o a H a r r i c k c e l l . 1 C w i t h a Haake c i r c u l a t i n g water bath and monitored w i t h a thermocouple p l a c e d as c l o s e as f e a s i b l e to the p o i n t where the IR r a d i a t i o n was focused. 2 2 2 ( FT-IR INSTRUMENTATION AND DATA ACQUISITION.
L. Biochim. Biophys. Acta 1977, 465, 260-74. 16. ; Chapman, D. Biochemistry 1980, 19, 349-56. 17. ; Jahnig, F. Phys. Lipids 1981, 29, 83-101. 18. ; Brown, E. Biochem. Biophys. Res. Comm. 1973, 54, 358-67. 19. W. Macromolecules 1973, 6, 708-15. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1990. 2. MENDELSOHN AND DAVIES CD Rocking Modes as Quantitative Probes 43 2 20. G. Phys. 1985, 82, 2811-24. 21. G. Chem. 1985, 89, 4390-5. 22. J. Statistical Mechanics of Chain Molecules; Hanser : Munich, 1989.
D a t a from Reference 25. The e f f e c t s o f c h o l e s t e r o l a t v a r i o u s b i l a y e r depths a r e shown i n F i g u r e s 6 and 7. F i g u r e 6 compares the CD r o c k i n g modes f o r 6 2 and 12-d^ DPPC a t 34°C, w h i l e the same molecules a r e compared a t 49°C i n F i g u r e 7. The r e s u l t s o f the q u a n t i t a t i v e a n a l y s i s a r e i n c l u d e d i n Table I I I . Most n o t a b l e i n F i g u r e 4 and Table I I I a r e the s u p p r e s s i o n o f gauche rotamer f o r m a t i o n a t 49°C a t the 6 ( 6 ' ) p o s i t i o n , as shown by the r e l a t i v e weakness o f the g t f e a t u r e s a t 1 646 and 652 cm" i n the c h o l e s t e r o l c o n t a i n i n g samples.