By G. B. Ansell
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Additional info for Variation in Chemical Composition of the Nervous System. As Determined by Developmental and Genetic Factors
Example text
C o l l i n s Cleveland P s y c h i a t r i c Institute, C l e v e l a n d , O h i o , U . S . A . The d i r e c t study of m y e l i n i s now p o s s i b l e due to r e c e n t a d v a n c e s in the i s o l a t i o n of m e m b r a n e p o t e i n - l i p i d c o m p l e x e s in pure f o r m by u l t r a - c e n t r i f u g a t i o n (CORNWELL and HORROCKS, 1964). Myelin was i s o l a t e d f r o m whole brain h o m o g e n a t e s by a s l i g h t l y modified method of LAATSCH, KIES, GORDON and ALVORD (1962).
After the a d m i n i s t r a t i o n of T r i p a r a n o l to patients b e f o r e s u r g e r y , a rapid a c c u m u l a t i o n of d e s m o s t e r o l in b r a i n t u m o u r s i s d e m o n s t r a t e d , while no d e s m o s t e r o l can be d e t e c t e d in the n o r m a l b r a i n t i s s u e of the s a m e p a t i e n t s . An a n a l y s i s of the c e r e b r o s p i n a l fluid of t h e s e patients showed that d e s m o s t e r o l tends t o a c c u m u l a t e in the CSF of m a n y of t h e t u m o u r - b e a r i n g s u b j e c t s .
In a n a e r o b i o s i s the quantitative ratio b e t w e e n g l u c o s e uptake and l a c t i c acid production i s about 0. 6 1 , In the e a r l y s t a g e s of a e r o b i o s i s it a p p e a r s to be a PASTEUR effect which r e d u c e s the g l u c o s e consumption by t h r e e to fourfold c o m p a r e d with that in a n a e r o biosis, 3) The uptake of L - g l u t a m a t e , u s e d a s s o l e s u b s t r a t e , w a s m e a s u r e d m a n o m e t r i c a l l y by using the e n z y m i c method of GALE (1945) with s p e c i f i c L - g l u t a m a t e d e c a r b o x y l a s e ( E .