By Harald H. Sitte, Michael Freissmuth
This ebook is a consultant survey of the present prestige of the constitution, functionality, legislation and molecular pharmacology of Neurotransmitter Transporters. It offers an summary of insights generated long ago 5 years. the quantity serves as an invaluable compendium of present innovations and an inspiring place to begin. it's a resource for college kids drawn to this rising box in addition to for knowledgeable scientists trying to find an replace.
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Additional resources for Neurotransmitter Transporters (Handbook of Experimental Pharmacology) (Volume 175)
Example text
1 Neurotransmitter Transporters Conduct Transmitters Across Membranes . 2 Methods to Study Transporter Function . . . . . . . . . . 1 Radiometric Assay . . . . . . . . . . . . . . . . 2 Electrophysiology . . . . . . . . . . . . . . . . 3 Amperometry and Cyclic Voltammetry . . . . . . . . . . 4 Quantitative Fluorescence Microscopy . . . . . . . . . . 5 Confocal Microscopy and Two-Photon Excitation . . . . . . . 6 TIRF Microscopy .
As seen in Fig. 7, for each pixel within the intensity image the corresponding fluorescence lifetime was determined. 5 ns). GFP has the same quantum efficiency as aqueous buffer and the cytosol; thus, no lifetime changes were observed. A solution calibration is directly applicable to cellular GFP. Similar values were determined from corresponding time-domain measurements (Fig. 8). The time-domain measurements demonstrate a multi-exponential decay, which AFUs to Molecules 45 shows that ASP+ decays from several excited states.
The resulting fluctuation can be analyzed in many ways, but the most common approach is by autocorrelation. Autocorrelation is a measure for the self-similarity of AFUs to Molecules 47 a time signal. The original fluorescence signal is translated in time by adding a set amount (∼10 µs) to each time point, without adjusting the corresponding fluorescence intensity. The resultant translated signal is compared to the original signal for correlation. The original signal is translated by an increased time (20 µs).