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These bands correspond to vibrational modes which involve motions of the atoms in the chromophore, that part of the molecule where the electronic transition is localized. Therefore the resonance effect provides a means whereby vibrations of biological chromophores can be distinguished from the many vibrational modes of a complex biological matrix. Resonance enhancement factors can be quite large, so that the chromophore vibrations completely dominate the spectrum, and can be detected at high dilution.

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