Metabolism and Metabolic Studies
Distribution of diet C is tissue particular with early saturation of the mind and adrenal glands following differential oral dose regimens in guinea pigs
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Additional info for British Journal of Nutrition (Volume 113, Issue 10)
6. Separation of total pig heart lipids by 3-direction TLC (57). 1. the major TAG fraction (98%) from the phospholipids is recommended using commercially prepared silica (62) or aminopropyl cartridges (63). Derivatization The most common derivatives used to analyze the long-chain alky groups of the different lipid classes are methoxy derivatives prepared by reaction with excess methanol in the presence of catalytic amounts of acid or base. Methoxy derivatives are preferred because of their greater volatility and superior resolution by GC.
The discovery that CLA protect against cancer (3) sparked great research interest resulting in an almost geometric increase in publications each year with involvement spanning multiple disciplines. Several additional effects have subsequently been observed in experimental animal and cell models attributed to CLA involving atherosclerosis, diabetes, obesity, and immune functions (4–6), as well as milk fat depression (7). The results of a few CLA isomers tested thus far suggest that the biological responses are isomer specific (4–6), but many CLA isomers have not yet been evaluated.
There are many different kinds of lipids, broadly categorized as neutral and polar lipids, with triacylglycerol (TAG) and phospholipids being specific examples of the two groups, respectfully. The first step in the analysis of lipids requires disruption of the cells followed by dissolving all the lipids in a suitable solvent. However, disruption of the cell exposes the lipids to the action of lipases that quickly Copyright ©2006 by AOCS Press Systematic Analysis of trans and CLA l 51 hydrolyze the different lipids to free fatty acids (FFA), and to oxidation.